In a groundbreaking discovery, researchers have developed technology that can help effectively treat heart disease in humans. The technology repairs cardiac muscles in mice after a heart attack and also successfully regenerates them. Researchers at the University of Houston used synthetic messenger ribonucleic acid (mRNA) to deliver mutated transcription factors to mouse hearts. Transcription factors are the proteins that control the conversion of TUSEN into RNA.
In their study, published in The Journal of Cardiovascular Aging, the team conducted an experiment to show that two mutated transcription factors, Stemin and YAP5SA, work closely together to increase the replication of heart muscle cells or cardiomyocytes in the mouse.
“What we’re trying to do is dedifferentiate the cardiomyocyte into a more stem cell-like state so that it can regenerate and proliferate,” said co-author Ph.D. Siyu Xiao. of the study. According to another co-author Dinakar Iyer, Stemin’s transcription turned out to be a game-changer in their experience. While Stemin triggers rod-like properties in cardiomyocytes, YAP5SA acts on organ growth resulting in greater myocyte replication.
Demonstrating the effects of Stemin and YAP5SA on the mouse heart, the researchers, in a separate finding, showed that the transcription factors repair the damaged heart. They observe that after the injection of the factors, the myocytes replicate at least 15 times in 24 hours.
“When the two transcription factors were injected into infarcted adult mouse hearts, the results were astounding. The lab found that cardiac myocytes multiplied rapidly within a day, while hearts over the next month were repaired to regain nearly normal cardiac pumping function with little scarring,” said Robert Schwartz, lead author of the study. ‘study.
According to Xiao, using mRNA in the treatment was better than vial delivery because it disappears within days. Moreover, when gene therapy is delivered to cells using viral vectors, it comes with some biosafety issues because it cannot be stopped easily.